Segregation of Numb protein during cell division in mammary stem cells. The spatial distribution of the polarity marker Numb (Green: Alexa 488; Cyan: DAPI) has been analyzed in normal (A) and cancer (B) stem cells. The 3D projection elaborated from a stack acquired with a Leica TCS SP2 confocal microscope clearly shows that the asymmetric partitioning typical of normal stem cells is lost upon transformation.
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