Shotgun Histology Brain

Shotgun Histology Brain

Category:

Howto & Style

Tags:

• Shotgun
• Histology
• Brain

[makanerwe]

awesome stuff.. helps me a lot to cram more knowledge for my exam!

[OTarO86]

Thanks ! This video is very useful to me but i still have the problem with the identification of other cell types in brain tissue like macrophage or PMN in the inflammatory response

[radium86]

@darlantro Just to add a little more explanation... The blood brain barrier was discovered by Ehrlich who injected dye into the blood and noted it didn't reach the brain, later one of Ehrlich's students injected dye into the CSF and noted the dye didn't reach the blood. (Much) more recent repeats of this second experiment with microscopy show that the endothelium of blood vessels in the brain, not astrocytes are the barrier, as the dye in the CSF reaches the endothelium but not through it.

[darlantro]

@Eklipse113 'astrocytes surround the blood brain barrier' is how I would describe the situation; I said I was splitting hairs with that point. (I have a doctorate in neuroscience, specifically related to brain injury).

[Eklipse113]

@darlantro From Pubmed: "Three cellular elements of the brain microvasculature compose the BBB-endothelial cells, astrocyte end-feet, and pericytes (PCs). "

I don't want to post a URL; the title of the page is "The blood-brain barrier: an overview: structure, regulation, and clinical implications."

This guy's been teaching this stuff for years... If you're a medical student like I am don't go assuming you know it better already.

[darlantro]

Cool video!

A lot of those small cells are endothelial cells forming vasculature... maybe worth pointing out that they are non-CNS but still in your slice. Also, there are smaller neuronal nuclei (for sure in layers 2,3 & 6of the cortex) so generalizing that all small cell nuclei in an area are non-neuronal is tough.

Related note; splitting hairs again, but astrocytes "are the blood brain barrier" is not entirely accurate, in that endothelial cells actually form the blood brain barrier.

[masinga6]

i thought arachnoid mater was avascular

[Dexterprog]

A little correction, the meninx is the dura mater, not the arachnoid

[noa440]

Great video. I love this series! Eminently useful for the neophyte pathology resident.

[firaselshufi]

what i know is that the arachnoid mater doesnt go deep inside brain fissures only pia mater does.arachnoid mater is attached to the inside of the dura.