Enzyme Immobilization: Nanobiotechnology: Putting Molecular Machines to Work

Describes, in general terms, the concepts of high-throughput protein expression coupled with immobilizations in functionalized nanoporous materials to carry out multiple kinds of diverse reactions. The animations also illustrate that immobilized enzymes potentially can refold inactive proteins. Transcripts of videos available upon request. Contact us at http://www.pnl.gov/contacts/feedback.asp.

Category:

Science & Technology

Tags:

• biomolecular
• systems
• science
• protien
• PNNL
• Pacific
• Northwest
• National
• Laboratory

[ContaMuir17a]

How do you shut it off? under controlled conditions in the lab it's not a big deal as long as the nano materials are destroyed when their job is done, but when these materials are released into the biosphere I'd be a little bit more concerned if there isn't a well defined and tested shut off mechanism.

[PNNLgov]

The first generation nanoporous materials are silica-based. The immobilized enzymes are inert unless they are presented with their substrates, so one way to turn off the enzymes is to simply not present them with fresh substrates or reactants. If it were necessary to destroy the immobilized samples, the silica would dissolve at high pH. For more information please contact eackermATsandiaDOTgov.

[Esceptik33]

buen clip sobre inmobilización de enzimas. Lo voy a traducira al español