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How to Run an SDS-PAGE gel

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Uploaded on Jan 23, 2010

Okay now that you know how to cast an SDS-PAGE gel, how exactly do you use it? Here, Dan shows you how to set up your apparatus to run SDS-PAGE.

WAIT!!! Don't have a gel? Watch this video to learn how to make it: http://www.youtube.com/watch?v=EDi_n_...

Some important points to remember:
- short glass plate faces inwards
- remove the comb carefully
- load samples inside the wells, and do not over fill (usually 10uL of sample is loaded)
- fill the inner chamber to the TOP with buffer
- fill the BOTTOM of the outer chamber with buffer
- attach power supply: RED to RED and BLACK to BLACK

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