RNA interference - RNAi
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Uploaded by mbrpointscom on Sep 4, 2007
This video shows how rnai works in the cells.
info ref: http://en.wikipedia.org/wiki/RNAi
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Yes, it's the same. The difference is earlier: miRNAs are first processed in the nucleus by Drosha - siRNA and shRNA (usually) are not. shRNA are ceaved by Dicer in the cytoplasm. siRNA resembles the product of Dicer. All three (miRNA processed by Drosha/Dicer, shRNA cleaved by Dicer, and siRNA) are about the same shape and size at this point and are all recognized by the RISC complex.
easternblot 4 years ago 2
Hmm... is the RISC holoenzyme the same for endogenous miRNAs and transfected siRNAs/shRNAs?
QuantumKitten2 4 years ago