DNA microarrays

A short film about DNA microarrays, and how they are used to show dynamic gene expression levels.

Category:

Howto & Style

Tags:

• DNA
• microarray
• gene
• expression
• chip
• bioinformatics

[befz88]

how do you know which one is infected and which wasnt if you mixed them before the array?

[Proneural]

You would typically have two different batches of cells, grown separately in culture. One batch of cells would then be infected, and after a period of time, both batches would have their RNA isolated and reverse transcribed and used for subsequent analysis.

[befz88]

And to isolate the RNA you would use phenol chloroform extraction? and then make cDNA from the RNA you collected?

[Proneural]

I use Trizol reagent to purify, which is similar to a phenol/chloroform precipitation. You just have to make sure that you eliminate genomic DNA, and DNAse digestion on RNeasy columns from Qiagen can do that. Then you can do the reverse transcription to make the cDNA.

Also, for RT-PCR, primers should be made to span an intron so that you can eliminate any possible signal from genomic DNA contamination.

[IsaacDLovesChrist]

@Proneural What is your process for designing the primers? Which company do you use to generate your RNA primers?

[planetxmapevent]

The registration for Planet xMAP is now open. The European multiplexing technology symposium in Vienna (28/29 Sept '11). Visit: planetxmap[.]com

[arepeace0687]

'@haz020190 Yes it is. The complementary strands were account as a whole genome.

[haz020190]

@arepeace0687 dont the complimentary strands in total just form the whole genome of the organism? from a gene library that was available?

[arepeace0687]

@boringgrass from what im understand, the complementary strand were predesigned by the researcher based on the target sequences of the viral mRNA (or cDNA preciesely) and were initially fix to the membrane.

[boringgrass]

at 0:37 where did the complementary DNA come from? how did they stick there?

[Ubuntu3011]

Much better than my book, thanks

[kirixnaru]

thanks!!! u helped me understand microarrays right before my final

[punkyv182]

9 people suck d*cks

[adbhutseleti]

Nyc animation...helpful stuff indeed!:)