Cesium Chloride Density Gradient Centrifugation

This is the technique used in the Meselson and Stahl experiment, which tested hypotheses about DNA replication. I explain how the cesium chloride density gradient forms, and where DNA goes in the gradient during centrifugation.

Category:

Education

Tags:

• dna
• density
• gradient
• cesium
• chloride
• meselson
• stahl
• genetics
• biology

[Jonway9]

So, if she weighs the same as a duck, then she's made of wood. And therefore... A WITCH!

[agathman]

@Jonway9 - Who are you who are so wise in the ways of science?

[Jonway9]

@agathman Oh I wasn't implying the explanation was flawed in any way. I was merely referencing Monty Python because your "even small rocks" comment reminded me of the duck scene.

[agathman]

@Jonway9 I know -- you didn't notice my response was also a quote?

[lmarcze11]

where does protein go in the gradient and why?

[agathman]

@lmarcze11 -- In the gradients that Meselson and Stahl ran, there would have been little protein there in the first place, as they were purifying the DNA before putting it in the gradient. Proteins should do the same thing that DNA or any other molecule does in the gradient, though. They would move to the place in the gradient where their density equals the density of the solution.

[mohammadrmit]

Clear explanation, keep it up

[Jonway9]

haha lol I missed that. You are too subtle for me :) I need to have a Monty Python marathon.

[innocentnavya]

Helpful!

[nemodot]

Great explanation!

[anupam15893]

superb best  and understandable video thanks alot.........