Make a spore syringe in 15 mins

This video shows the basic method to how a spore print is converted or made into a spore syringe. This video shows a method not sterile, but coveres the method for use in a sterile glove box.

Category:

Howto & Style

Tags:

• mushroom
• spore
• syringe
• mycology
• fungus

[flashtin166]

To store spores is it better to store in the syringes or just store the prints?

[TeranTula1967]

@flashtin166 Spore prints store longer than syringes.

[modernrocks]

1Q.In treory could very few even one spore produce never ending copious myco?

also 2Q. Isnt distilled water sterile, does it need boiling?

[TeranTula1967]

@modernrocks 1A. No, not even in theory. You would need at least two spores of opposite sex to form clamp connections and exchange genetic material before any mycelium that would form mushrooms would be formed. After the mushroom is formed, then yes, an infinite supply of spores equal to the enviromental conditions provided.

2A. Distilled water is NOT sterile, and does need to be "Pressure Treated" to become fully sterile.

[opalweiss]

This Guy Is GOOOOOOD

[BornAgainAtheist]

GREAT VIDEO.

Thanks a lot.

Is that an egg carton you've got there holding all of your print's and syringes?

Good idea if so, if not I just had a good idea!

[TeranTula1967]

@AIienware This is all moderated by a process called "Sectoring". Where you take a small genetic sample for culture on nutrient agar, the rest is used in actual fruiting culture. Once the Biological efficiency (how much of the food is converted into mushrooms) is determined, the culture is then evaluated for further culture. If it cultures well, then the original agar plate is noted as a "Master" for further culture, and only the smallest of amounts are ever used in mycelium expansion.

[TeranTula1967]

@AIienware Now technically, "Life Finds A Way". In some very VERY RARE cases, the two different strains will form clamp connections and actually exchange genetic material. Essentially, instead of fighting for food, they breed, essentially becoming "one mycelium patch". When I use more than one print (or portions of prints), I use the same strain but different breeding cycles, and I do this to attempt reintroduction of genetics that will hopefully assist in increasing overall viability.

[TeranTula1967]

@AIienware Mixing prints, yes and no. If they are the same sub-strain yes, otherwise no. Same strain/species mixes are often used to increase the viable genetic base of a culture. NOTE HOWEVER, Differing strain/species causes the mycelium to waste energy on combating other competitor species and is not advised. Typical commercial sale of a spore print syringe will have a ratio of one print to 20 or more syringes, and some companies even stretch this out to 50 syringes or more(bad). I use 1:1.

[AIienware]

can u mix different spore prints? and how many syringe can you filled up for 1 print?

[ordnaelcabral]

@TeranTula1967

Thanks a lot !!!Peace



[TeranTula1967]

@picaticatara True. An entire culture library of spores and living mycological samples can be obtained from both commercially available strains as well as wild finds.