Cadiomyogenesis of human mesenchymal stem cells

Marrow-derived human mesenchymal stem cells (hMSCs) have short proliferative longevity, compared with embryonic stem cells, and thus, important challenge in regenerative medicine is to improve the replicative capacity of human hMSCs. The life span of hMSCs have successfully been prolonged by bmi-1, which reduces p16INK4a expression and is essential for self-renewal of hematopoietic stem cells. The hMSCs with a prolonged life span began spontaneously beating, exhibited having action potential of cardiomyocytes, and acquired automaticity. Based on the mechanism of cell life span extension, large number of hMSCs can be obtained sufficiently to improve cardiac function of heart disease.

Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation? Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Hida N, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, Umezawa A. J Gene Med. 2004, 6(8):833-845.

http://www.ncbi.nlm.nih.gov/pubmed/15293342?ordinalpos=2&itool=EntrezSyst...

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