Gel electrophoresis

Setting up and running of a DNA gel using electrophoresis.

Category:

Education

Tags:

• DNA
• Biotechnology
• lab
• electrophoresis

[1006Will]

Hi Questions:

you made two sets of wells in a single gel, how did you know that the fragments from the upper wells won't travel THROUGH and hit the second row of wells?

secondly, how long does the whole process take?

[gkpeter]

@1006Will You can monitor it by keeping track of the migration dyes. In the video, you will see there are two colored dyes. The fastest moving one runs at about 500 base pairs. So, if you know the sizes of your DNA, you can prevent it from running into the next set of wells.

This whole thing can be done in 1.5-2 hours. Depends on your conditions and number of samples.

[ZScarabello]

Hi gkpeter,

I was wondering if you or anyone else here could explain to me why there is a need to identify sizes of DNA. What is the point of this exercise? Can you extract the DNA after it has been separated?

[gkpeter]

@ZScarabello There are lots of reasons to know the sizes. Verification of constructs, genotyping, cloning, etc. But the simple answer is, yes, you can extract the DNA from the gel if needed. I have done it several times.

[eyeslikestars001]

We are just learning about this in my AP Biology class, and this video helped me understand it a lot! Thanks! :)

[ronaldo204100]

Great video, it helped me a lot during lab!

[kyywii]

Damn i so glad i worked in a lab over the summer, otherwise i'd be screwed right about now

[CiaoBello21]

I once accidentally destroyed the agarose gel filling in my last sample, simply because i punctured the gel with the tip. Whats even funny is that i had a group of 5 others who were watching me as if their life depended on my hands.

[fappingfrenzy]

lol shaky hands

[luar2521]

thank you for this video

[Lupucillo]

Looks like Smurf jizz.

[BigBoss7777777]

@gkpeter Hi, I was just wondering what DNA gel is used for? Is it used in skin care products?