Targeted Gene Integration Part 3 of 3

Third chapter of our ZFN webinar series: Targeted Gene Integration
To see our other webinars, visit http://bit.ly/ZFN_Web1
For more information on CompZr® Zinc Finger Nucleases visit http://bit.ly/compoZr_YouTube

It's a revolution in genomic science. A technique capable of altering the face of basic research and drug discovery. And it's here, ready to help you unlock the darkest mysteries of the scientific universe.
CompoZr® ZFN technology is a breakthrough that enables simple and efficient genomic editing exclusively from Sigma Life Science. Zinc Finger Nuclease (ZFN) technology, allows easy creation of novel cell lines and model organisms with precise and heritable gene additions, deletions or modifications.
CompoZr ZFN technology gives you the power to add, delete or swap your gene of interest. Now it's all possible.

The details:
Zinc finger nucleases (ZFNs) are a class of engineered DNA binding proteins that facilitate efficient targeted editing of the genome by creating double-strand breaks (DSBs) at user-specified locations. The cell then employs the natural DNA repair processes of either homology-directed repair (HDR) or non-homologous end joining to heal the targeted break. HDR enables insertion of a transgene or other defined alterations into the targeted region. By this approach, a donor template is used that contains the transgene flanked by sequences that are homologous to the regions either side of the cleavage site. This donor is co-delivered into the cell along with the ZFNs. Target integration can be performed at user-specified genomic locations - including transgene expression from safe harbor loci. Utilization of a safe harbor locus for targeted integration enables stable expression of the transgene without adverse effects arising from the site of integration with decreased experimental variability. ZFNs are also used for gene tagging whereby a marker, such as GFP, can be fused directly to the endogenous gene. These types of experiments are used for the study of gene expression and cellular localization of the protein. Additionally, ZFNs can be used to recreate or correct disease--related mutations at the endogenous locus. In this webinar, we will discuss the various applications of gene integration.

Category:

Science & Technology

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• transgenics
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• knockout
• knockin
• genomics
• functional
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• Collingwood
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