Enzyme function and inhibition (with audio narration)

This was created over a couple of days to demonstrate how a simple animation can be used as a learning aid for scientific concepts that may be difficult for a student to grasp. The audio came out quieter than expected, so you may have to turn up your speakers slightly.

In the above animation, the normal enzyme catalytic cycle is shown and is followed by two types of inhibition by competitive and non-competitive blockers.

Feel free to contact me if you have any questions or comments.

Category:

Education

Tags:

• enzyme
• inhibition
• catalysis
• science
• animation
• enzymatic
• catalytic cycle
• tutorial
• help
• inhibitor
• inhibited
• competitive
• non-competitive
• function
• lesson
• school
• grade
• physiology
• biology
• microscopic
• explanation
• cycke
• catalytic

[Kamillafri]

hmmm I am confused, your description of competitive inhibition soulds to me like non-competitive inhibition and your non-competitive like allosteric inhibition..

[JubbaTheHott]

@Kamillafri allosteric inhibition IS the binding of something to the non-active site of an enzyme causing a change in the shape of the enzyme's active site - which is ALSO non-competitive inhibition since it's not competing with anything for use of the actual active site. i don't know why you think competitive inhibition seems like non-competitive inhibition, though...if two things are trying to bind to the same active site, they are competing for it....

[jeydii06]

Very informative. Albeit the speaker talks too fast. I need time to process what you're saying, man. lol. Thanks for this great vid though! Best one out of five I've seen so far.

[JubbaTheHott]

@jeydii06 thanks - i animated first and tried to fit my voice in after...next time maybe i'll slow down or loops my animation so i don't have to talk quite so fast.

[Measonz]

This video is very helpfull

[JubbaTheHott]

@Measonz thanks, glad it helped!

[zacamla]

this is going to help me so much on my bio final exam in a couple days, thank you!

[juzforjokez]

helped me, thanks

[zed2016]

woow it took me a minute to understand this compared to the 2 classes of notes i took and yet i was confused dayuuum

[slycordinator]

@JubbaTheHott "allosteric inhibition IS the binding of something to the non-active site of an enzyme causing a change in the shape of the enzyme's active site"

That isn't necessarily inhibitory. As far as I've read, there are some enzymes that function by one substrate attaching to an allosteric site which then causes the active site's shape to change such that it now can fit for the active substrate. :)

[wlrsruss]

The depiction of competitive inhibition is good but the non-competitive inhibition is wrong. A non-competitive inhibitor does not prevent substrate from binding, rather it prevents catalysis from occuring, so overall Vmax decreases but Km is unchanged. Also the type of Inhibition (competitive, non-competitive, mixed or uncompetitive) is defined by the kinetics NOT by how or where the inhibitor interacts with the enzyme.

[9tify1]

nice video....umm i was wondering what is irreversible inhibition then?...m guessing that the blocker attaches to the non-active site causing the enzyme to change its shape permanantely..please let me know if i am right...thnq

[CowGalz]

This is going to help me do my bio assignment ! the bitch is going to ask me for it 2morrow !

[wearesilver22]

thank you! nice voice btw ;)

[DiamondtheSimon]

Just some last minute study before my bio exam tomorrow. Cheers for the video.

[vidusha123]

Short n Sweet.....thanks it helped very much.... ;)