Confcoal imaging with Leica TCS SP5

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Uploaded by on Jun 17, 2008

Specimen: danio rerio
Treatment: RNAinj
Fishline: WT/TU x WT/Tu
Microscope: Leica TCS SP5 Confocal and Multiphoton Microscope with conventional scanner
Excitation: t-Pulse Amplitude 1030nm +980 (mai tai)
Objective: 20x/0,95NA W Olympus
Zoom: 1x
Room temperature: 22 degrees C
Speed: 200 Hz
Channels (n, g): 0,1 averaging

Imaging scheme:
Voxel: 1.37 x 1.37 x 1.37 μ³ Type: L4
Volume: 512x512x139 px³ (420 stacks)
699.9x699.9x189.06 μ³
Duration: 20:39:59 h 640 (2:57 min 142 between stacks)
Stage 0: between heigh and sphere
End stage: 17 somites

Operator: Nadine Peyrieras

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