Gram Stain Procedure

The steps required to do a Gram stain (Microbiology). Decolorizer used is 5% HCL and 95% Ethanol

Wife was a little upset about the sink!

Category:

Howto & Style

Tags:

• gram
• stain
• gram's
• bacteria
• slide
• iodine

[tuddyfruity4]

my husband and I do gram staining at home for fun

[spacewolf5462]

@ElNinoLalibre, I'm only 5 months too late, but I'll tell you the difference anyway

Gram Positive bacteria have a thick wall of peptidoglycan. When you add the mordant (grams iodine), you crystalize the crystal violet and basically get it stuck in the cells so that it won't wash out.

Gram negative bacteria have a tiny amount of peptidoglycan, so the alcohol wash actually breaks holes in the cell wall. This means the crystal violet will leak out of the cell. All thats left is the counterstain.

[alm7benc0m]

@alm7benc0m

من وين ؟

[jameeljo]

@alm7benc0m :)

[alm7benc0m]

@jameeljo

(:

[jameeljo]

I wish you showed us how the drying by heat is performed!!!

[jameeljo]

@alm7benc0m HOw can I like something that I haven't tried?

[alm7benc0m]

this is site good in lab

laboratory-testing . org

please like

[Rojjergeil]

It is a good video to teach the students. its a fact that the things we have seen, become more rememberable n so this video also playing a same role.........

Rajesh M.S.

[geezeweezebabypls]

Bloody procedure



[eyebal]

@kingofweightloss

Ok, but what about the endotoxins produced by the gram negative bacteria?

OHMYGOSH

[renesisrx8]

I think I keep washing off the bacterial smear every time I do it. I can't see anything under the microscope. I heat fixed the bacteria on the slide but as soon as I rinse off the crystal violet, the slide looks like it's been washed clean. The smear isn't visible anymore. I get to try it again tomorrow so I will try to leave the crystal violet on a bit longer and maybe add 2 or more loopfuls of bacteria to the slide.