Farewell to the Other Half: Holliday Junction Resolvase

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Uploaded by on Feb 14, 2008

In homologous recombination, the four-way (Holliday) DNA junction is the central intermediate. To produce the daughter DNA strands, this junction is cut by junction-resolving enzyme. In this video, a synthetic four-way DNA junction is in complex with phage T7 endonuclease I (2PFJ.pdb: J. Hadden et al. Nature vol. 449, 621).

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