Jurkat T-cell making contact with a Raji B-cell

An animated SFP rendering of a deconvolved time series. The original data was imaged using a Zeiss Cell Observer HS system with a 40x Fluor 1.3 oil lens and deconvolved using Huygens Essential. The original time interval is 1 minute.

"We look at the molecular events and redistribution of certain markers for T-cell activation like the TCR (green) and Actin (red). In the time series a ring-like pattern of Actin and a bulls-eye pattern of CD3 can be seen, accumulating at the contact-site (the immunolgical synapse). Both structures gave us a hint that the T-cell was activated properly."

Recorded by Christian Junker M.Sc., Institut für Biophysik, University of Saarland, Germany.

Category:

Science & Technology

Tags:

• raji
• jurkat
• t-cell
• b-cell
• deconvolution
• volume
• rendering
• 3d
• microscopy
• sfp