DNA REPLICATION

This movie is made by Dr Nguyen Thanh Cong working at the Agricultural Genetics Institute in Hanoi, VIETNAM
Address below:
Dr Nguyen Thanh Cong
Molecular Biology Laboratory
Agricultural Genetics Institute
Vien Di truyen Nong Nghiep
Tuliem, Hanoi
VIETNAM

Category:

Howto & Style

Tags:

• DNA
• REPLICATION

[5amGordon]

its a matter of understanding the big words.

[gatorpower]

The most interesting part about DNA replication is what happens on the lagging strand. That's not even covered in this video :(

[malbert34]

Simple explanation of DNA replication is as follows:

1. DNA helicase unzips the DNA Double Helix into two separate strands.

2. DNA polymerase adds free nucleotides to the complementary base pairs.

3. Hydrogen bonds form between the complementary base pairs.

Double helices are semi-conservative because they each helix is made up of one new and one old strand of DNA.

[bboydjoe]

@G1ingy cuz u need to synthesize the new strands from 5'->3'. so DNA pol III attaches to the 3' end of the template, and goes from 5'->3'. when it reaches the fork, it must also go from 5'->3', but since the 3' end of the other template is at the fork, its hard for DNA pol III to attach there and do what u said, so it must be formed in fragments.

[MegaSerena19]

Perfect VDO!!!!! :D

[G1ingy]

I still don't get it. Why does one have to be made from okazaki fragments while the leading strand can do it in one go? Why not both use okazaki fragments or both use DNA polymerase to do it in one go?

[samsta001]

@Msholyangel17 DNA synthesis of the new strand is 5' to 3' so it would be added to the existing strand from 3' to 5'.

[hifatpeople]

@gatorpower since when is it interesting at all? unless your making X-men

[Msholyangel17]

Please help here? I am so confused. DNA synthesis is 5' to 3' but the new template made is 3' to 5' right? What about the nucleotides being added? Which direction does that go? The nucleotides are being added to the existing strand in 5' to 3' but after it is added and sealed by ligase, the new strand is antiparallel which is 3' to 5' right? SO, if I have a question which asks the nucleotides are being added in what direction? What is it? 5' to 3' or 3' to 5'???

[Fairieswell]

Just want to add this. DNA Polymerase I is the enzyme that replaces RNA primer with DNA nucleotide.

[AQhockey44]

these videos are very helpful, except im still having trouble understanding transcription

[icequeenfirefly]

@Dressagebabie16 well i belive god created the beggining bacteria and then that developed into early speicies and then eventualy, us. i don't believe just one is true