Spectrophotometer Demonstration
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If you're looking for calibration kits check out NSG Precision Cells
flibberswooper0 3 weeks ago
Robot
TheLittleTrickster 3 weeks ago
@superphotonic The machine may be warming up the liquid increasing the kinetic energy of the particles and their rate of collision (not sure if that's the correct terminology for absorption....) with the rays of light, blocking more of them and increasing absorption. I'm just guessing here so you should probably ask someone that can give you a definite answer but I'd imagine a reading taken sooner rather than later would be more acurate
scruffles09 1 month ago
Should I wait a particular amount of time between placing a cuvette with the sample in the spectrophotometer and taking the absorbance reading?
It seems the longer you wait, the higher the absorbance reading becomes...I've been wondering about the significance of this for a while, and what absorbance reading is the "correct" reading, so to speak?
superphotonic 1 year ago