( http://www.abnova.com ) - PCR cloning is a method of cloning which dramatically reduces the time and effort put into the cloning reaction. PCR cloning procedure consisting of the four following steps: (1) production of a fragment of the gene using PCR, (2) digestion of genomic DNA, (3) ligation into a plasmid vector, and (4) transformation into bacteria and then bacteria will replicate the plasmid. More videos at Abnova http://www.abnova.com
it's very similar to my work.
wantanatan 1 month ago
Cool video!
mustardofdoom 3 months ago
@iarue You mean an electrophoresis machine? In this case one isn't needed (yet) because they aren't separating different sequences for comparison.
mustardofdoom 3 months ago
So GOOD!!!!
cimuscw 4 months ago
cool channel.
do you have videos with restriction enzymes and ligation on vectors?
im stuck in this area. please.
thnks
CientificoDeista 4 months ago
Sorry was that pCR cloning or just DNA cloning. Because no PCR machine was used
iarue 4 months ago
very nice music too!!
chrisvinu 8 months ago
this was really good thanks
youtubyfein 1 year ago
thank you : )
proteinP53 1 year ago