PCR Cloning

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Uploaded by on Mar 30, 2010

( http://www.abnova.com ) - PCR cloning is a method of cloning which dramatically reduces the time and effort put into the cloning reaction. PCR cloning procedure consisting of the four following steps: (1) production of a fragment of the gene using PCR, (2) digestion of genomic DNA, (3) ligation into a plasmid vector, and (4) transformation into bacteria and then bacteria will replicate the plasmid. More videos at Abnova http://www.abnova.com

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Science & Technology

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  • it's very similar to my work.

  • Cool video!

  • @iarue You mean an electrophoresis machine? In this case one isn't needed (yet) because they aren't separating different sequences for comparison.

  • So GOOD!!!!

  • cool channel.

    do you have videos with restriction enzymes and ligation on vectors?

    im stuck in this area. please.

    thnks

  • Sorry was that pCR cloning or just DNA cloning. Because no PCR machine was used

  • very nice music too!!

  • this was really good thanks

  • thank you : )

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