Northern Blot
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Uploaded by ABNOVA1 on Jan 21, 2011
( http://www.abnova.com ) - The northern blot is used to study gene expression by detection of RNA (or isolated mRNA). A general blotting procedure starts with the separation of RNA by electrophoresis. The RNA can then be transferred to a nylon membrane through a capillary system and detected with a hybridization probe. More videos at Abnova http://www.abnova.com
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Hi. I have a question regarding the Northern and Southern Blot.
I have watched both videos of ABNOVA1, and I can't really tell a difference.
Are the only differences in both techniques the solutions and the material(DNA/RNA)? I myself am a 3rd year college student, studying to become a technician in biochemsitry at Zuyd University in Heerlen, the Netherlands, and for my interim I'm supposed to do PAGE, western, eastern and southern blots.
Regards, Vexan
vexanalasea 4 months ago