3D Reconstruction of Frozen Plant

For the anaglyph version of this video go to: http://www.youtube.com/watch?v=d0gHElvObJM

There is no sound in this video

This is an example of using color keying to digitally clear plant tissue and allow visualization of internal structures in 3D. For another example see Arabidopisis flower in 3d at: http://www.youtube.com/watch?v=zlw-50RHoec&feature=youtube_gdata . The technique is ideally suited for in situ hybridization analysis to detect mRNA in an anatomical context

This is not a cartoon. This 3D volume was reconstructed from JPEG photographs of individual slices of plant tissue stained with Safranin and Fast Green. Photos were taken with a consumer-grade camera (Sony DSC707) mounted on a Nikon Eclipse 50i microscope using a 4X plan apo objective.




JPEG images were imported into Adobe After Effects where they were aligned and the 3D volume was constructed. The full HD version is ½ the full resolution available. When the technology catches up, we will re-post at higher than 1080.

Distortion-free sections were prepared by Research Associate Tan Tuong. Graphic consulting was provided by Randy Gelman, BeltlineMedia, http://www.beltlinemedia.com

For more information see Livingston et al 2010. 3D volumes constructed from pixel-based images by digitally clearing plant and animal tissue. Journal of Microscopy. PDF was on my website at:: http://www.ars.usda.gov/Main/docs.htm?docid=19570, but video embedded in PDF did not work. Will post soon.

Contact: David Livingston, dpl@unity.ncsu.edu 919-515-4324

Tags: anaglyph" '3D" "red-green glasses" histology "in situ hybridization" "Nikon Eclipse 50i" "Adobe After Effects" "digital clearing" microscope "JPEG images" "serial sections" "frozen plant" "oat crown"

Category:

Science & Technology

Tags:

• histology
• in situ hybridization
• Nikon Eclipse 50i
• Adobe After Effects
• digital clearing
• microscope
• JPEG images
• serial sections
• frozen plant
• oat crown