Dendritic Exocytosis of TfR-pHluorin Following Glycine-Induc

TfR-pHluorin fluorescence was imaged before and after glycine stimulation. Arrows and arrowheads indicate exocytotic events in growing and newly formed spines throughout the dendrites, respectively. The duration of glycine applied is indicated by the white circle. A pseudocolor intensity scale bar is shown. Time is indicated in min:sec. The images are 30 μm wide.

Category:

Howto & Style

Tags:

• LTP
• spines
• plasticity
• exocytosis

[pjdude]

So basicly glycine is stimulating new dendrite groth on a neuron? And a TfR-pHluorin is the stain being used? I guess the idea is that you could use this stumulus to form new pathways... I'm just guessing here.

[amnesticgnxp]

the whole thing is a dendrite.. the things that are growing are called dendritic spines.. yeah TfR-pHluorin is a marker for new chunks of cell membrane being added so as to grow the spines more..

[drtreg]

It is still a brilliant video of how dendrites are moving all of the time and probably the best video on Youtube for dendrite spines. Are there any more dendrite spine/ dendrite videos elsewhere or coming up on Youtube? I cant seem to find them anywhere else on Youtube. Dendrite spines are the new black.

[Neuroslicer]

The problem with using 2 D imaging to show the appearance of a 3 D structure is that it is conceiveable that each of those dendritic spines was initially hidden from view, and that during the long course of your imaging session, these spines moved into view. If you look at each of your spines indicated by the arrows, I see some signs of movement at those sites before glycine treatment.

[pixmusic]

sorry for my skepticism but your signal doesn't seem like exocytic events of TfR-phluorin after Gly stimulation. usually these are seen as sporadic big bursts. One possible problem is the low sampling rate, other the Gly LTP protocol itself (which is hard to accomplish itself). Check the Park et al paper (Neuron 2006) for a better idea of what these look like.