polymerase chain reaction Animated

it is easy to understand

Category:

Education

Tags:

• animation
• of
• polymerase
• chain
• reaction

[JackBeNimblest]

What happens if you're missing one Amino Acid or have a AA-Pool that is very irregular?...

[zubair908]

@JackBeNimblest see im also a student..... nt teacher.... so im sorry dude...

[cubixguy77]

Even taq is shaking to the beat!

[KostaLaskaratos]

enzymes are fucking crazy, Creation baby. Fucking Creation, this Vid is turning me on.

[ilhsfm]

this song makes PCR so much more epic haha

[runningbladez]

This makes my life so much easier to understand it :) thank you

[piarocio2013]

@JackBeNimblest We waatch your video all the time in biomedical science class! lol thanks for making it easy to understand. :)

[tarabowers1]

@JackBeNimblest concentration of the "amino acid" pool is actually a pool of dNTP - or nucleotide precursors, if there is an irregularity in these pools you may end up with mutations or mistakes

[PND3M1KR3V0LUT10N]

@PND3M1KR3V0LUT10N If the protein doesnt fold right, it doesnt work right, and problems start at the cellular level, which can be compounded by other parts of the cellular machinery not working in response to the first malfunction, which can cause issues at the organismal level.

Cystic Fibrosis is an example of a disorder caused by a mutation in the gene for a type of chloride channel protein in the lungs.

[PND3M1KR3V0LUT10N]

@JackBeNimblest Sickle Cell Anemia, Muscular Dystrophy are examples of non-cancer disorders that arise from mutation in a DNA sequence. Sickle Cell Anemia comes from the replacement of one codon with another, typically by the replacement of one base in the three-base codon sequence (i.e. DNA : ATT --->CTT corresponds to AUU ---> CUU RNA sequence which would replace an isoleucine with a leucine (Amino acid) The way the R groups of AA's interact determine how the protein product folds & functions

[PND3M1KR3V0LUT10N]

@JackBeNimblest @JackBeNimblest If youre missing an amino acid, translation is affected, not replication. If you meant "missing a (dNTP or nucleotide) then youll have incomplete replication of the target DNA sequences. You will get far fewer copies of the DNA because once the missing dNTP comes up in the replication sequence, replication cant continue. That missing or low-concentration dNTP is the limiting reagent in the reaction. Missing codons = mutations~~>cancer or other disorders

[arkhumrox]

@JackBeNimblest I think they put an excess of amino acids just to be safe, but that's just a guess :)