Chromatography Observed at 128x Speed!

This is a chromatography column that I did in Organic Chemistry lab. You can watch β-carotene and lycopene(to a lesser extent) travel through silica gel to be collected at the bottom.

Two solvents were used: hexane and a 9:1 mixture of hexane and ethyl acetate. The hexane drives the β-carotene down the column, then the 9:1 was added. Due to the polar nature of the 9:1 and lycopene, the red band begins to move more quickly upon the 9:1's addition. This can be observed in the video.

An hour of work for 40 seconds of video? It was worth it.

Category:

Entertainment

Tags:

• Lycopene
• β-carotene
• beta
• carotene
• organic
• chemistry
• lab
• Derek
• Thacker
• UAH
• University
• of
• Alabama
• in
• Huntsville

[thacked]

Oh and trust me, you don't wanna see the normal speed vid..it's like 48 minutes long.

[thacked]

the song is "scream out" by the unseen. I give credit to them at the end, though you maybe can't read it.

Hmm, perhaps you're correct about the polarity. I'd check if

1. organic wasn't done for the semester

2. it wasn't 5:30am with me still having not been to bed; my chemistry skills fade late at night:)

Thanks for the comments though!!!!

[darkcurrent]

What a shitty column run you did.

The eluent is clearly not perpendicular to the column. You either did a shitty job packing the column or loading the column.

Next time, cap the column and pump air into the column so you won't have to speed the run by 128x. You also get less band broadening.

n00b.

[raydogg969]

@thacked 16x speed would have been enough though

[fugert]

fuck yeah!!!!

btw has anyone really been far even as decided to use even go want to do look more like?

[agrippaviar]

omg shitty column no resolution at all -_- dont upload trash ok? sigh

[TragicAntiHero]

i remember my β-carotene travelling first and i ended up "not needing" the 9:1 solution. "what you add to this?" "this... is not right." vogler ftw.

[AnneRush87]

I love Derek :-)

[midimaster82]

dude, i think u meant lycopene is less polar. less polar travels faster in a silica gel column. unless if u'r using reverse phase silica gel.

[musiclover709394]

it's a great video but can u show a experiment at normal speed.......??

by the way zxab the bg music is awesome, what s that song ?