New-born exitatory neurons in cortex - trangenic mouse
excitation: 910 nm
imaging depth: 400 microns
BAC transgenic Eomesodermin:EGFP (GENSAT) mouse pups at postnatal day 4 were perfused with 4% paraformaldehyde. The dorsal cortex was dissected and post-fixed overnight in 4% paraformaldehyde and stored in PBS. The GFP reporter labels a sub-population of excitatory neurons in the cortex.
Movie acquired with Leica TCS SP5 MP - DM6000 CFS
Courtesy fo Dr. Ashkan Javaherian,
Kriegstein Lab., UCSF-Insitute for Regeneratiion Medicine,
San Francisco, CA, USA
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