Indirect ELISA

( http://www.abnova.com ) - The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample. In the assay, the antigen of interested is immobilized by direct adsorption to the assay plate. Detection of the antigen can then be performed by using a matched set of primary antibody and conjugated secondary antibodies. More videos at Abnova http://www.abnova.com

Category:

Science & Technology

Tags:

• Indirect
• ELISA

[ABNOVA1]

We don't have name for background music but we like it as well. Thanks for your support!

[MalagaJolu]

Ok, for example I have my purificated Ag ( I want to perform a indirect ELISA) in Tris buffer 50 Mm, 100Mm Kcl, do u recomend me hibrid my antigen to the microplates with that buffer?

[MonsieurLavabo]

@0102murphy No, the worst scenario would be cracking your plate if you're hitting extremely hard (which is unnecessary) but it is very unlikely for the Antigen/antibody bonds to break.

[MonsieurLavabo]

@MalagaJolu No, it really depends on the assay you are doing. For example it can be an antibody in Blocker Casein/PBS.

[MonsieurLavabo]

Holding the pipette vertical and pre-rinsing the tip will help you get more accurate results and reduce the variation between wells.

[MalagaJolu]

the coating buffer allways have to be 0.1 M Carbonate pH 9?

[0102murphy]

Will the coated antigen in the plate being shaked out if you hit the plate against the table so vigorously??

[PreMedReview]

Haha, weird question but what is this song called? Its really nice! Excellent video series on molecular techniques by the way!