So, does adding Bromophenol blue to the stacking gel affect the polymerzation in any fashion? What about it focusing the samples into their bands? This video made me think, why can't we use B.B. in all stacking gel in order to see the wells more clearly? I'd ask my PI but sheesh....don't wanna change his tradition, so I'll ask you first!
hey, this is so nice! adding B.B is new to me too... what's the concentration added to the gel? thanks
lisasim 2 years ago
So, does adding Bromophenol blue to the stacking gel affect the polymerzation in any fashion? What about it focusing the samples into their bands? This video made me think, why can't we use B.B. in all stacking gel in order to see the wells more clearly? I'd ask my PI but sheesh....don't wanna change his tradition, so I'll ask you first!
xxyanonyxx 4 years ago