Bacteriological Three-Phase Streak-Plating Technique

Here is one of probably dozens of methods by which a plate can be streaked such that one can spread out the colony-forming units to result ultimately in well-separated colonies. Naturally this takes practice.

For ease in seeing the action, the lid is left completely off when we show the three phases being streaked - something we would never do in our teaching laboratory. Also, one may need to utilize personal protective equipment ("PPE") and/or work in a safety hood (provided with laminar airflow) depending on the Hazard Group to which particular species are assigned.

Macintosh OS 9 "Victoria, high-quality" voice is used for the narration as it reads and pronounces the text better than the inferior OS X version. (And certainly better than mine.)

Category:

Education

Tags:

• bacteriology
• microbiology
• laboratory
• technique

[pollo1m]

good video!

[johntubeseven]

Thank you!  The air in our teaching lab tends to have a very high contamination potential so we "keep the lid on" as much as possible.

[microbiologistsc]

@johntubeseven it should be made in laminar

[joe404WTF]

Sounds like the computer in Half Life Portal :)

Aperture Lab Rulez!!! :D