How to set fermentation reaction in a fermenter (Part 1).MPG

Any fermentation process can be carried out as Batch, Continuous and Fed Batch.
1. Batch: fermentation process allowed proceeding for limited time with required controlled conditions. After the stipulated incubation period the process stopped and yield recovered
2. Continuous: fermentation process allowed to proceed continuously with continuous supply of required nutrients and sample withdrawn regularly
3. Fed batch: fermentation process allowed to proceed continuously and sample withdrawn discontinuously with intermittently addition of medium
Various components of an ideal fermenter for batch process are:
1 .Top -plate cover (made of steel)
2 .Clamp -top plate compressed onto vessel using clamp
3 .Seal -separates top plate from vessel (glass) to prevent air leakage
4 .Vessel glass, jacketed, steel with ports for various outputs, inputs, probes
etc
A silicone tube is fixed onto the outflow cooler on one side and onto the corresponding filter on the other side. The air outflow cooler is now screwed into the side neck of the Fermenter vessel.
The large silicone plug is screwed, together with a washer, to the larger side neck using the screw-hole cap. If necessary lock end pieces are mounted onto the top of the cannulae. The long cannula is preferably used for sample collection, the shorter cannulae are used for addition of pH-adjusting substances and inoculation.
5 .Drive motor used to drive mixing shaft
6 .Drive shaft mixes the medium evenly with its impeller
7 .Marine impeller for plant tissue culture
8 .Baffles- prevent sedimentation on sides and proper mixing
9 .Sparger air supplier / after filtration via membranes -- ensures efficient
dispersal -- by attached to impeller
10 .Exit gas -cooler like condenser remove as much moisture as possible from exhaust
11 .Inoculation needle-port to add inoculum
12 .Feed pumps- regulates the flow rates of additives (medium, nutrients) variable speed
13 .Peristaltic pumps -fixed speed pumps -- used for continuous sampling
14 .Syringe pump -using a syringe -- mostly used in batch
15 .Exit gas analysis- CO2 analyzer, O2 analyzer, mass spectrometer
16 .Sample -pipe through which samples are drawn
17. 3 way inlet to insert different probes

Category:

Science & Technology

Tags:

• Microbial
• Fermentation
• Technology