Hippocampal Neuron in 3D rotation

A movie of a cultured rat hippocampal neuron reconstructed in 3D using pseudoconfocal microscopy (deconvolution by Slidebook [Intelligent Imaging Innovations, Inc., Denver, CO]).
Color Key:
Green = cytoskeleton; red = synapses; blue = nucleus.

Category:

Science & Technology

Tags:

• Neuroscience
• neuron
• brain
• hippocampus
• synapse
• rat
• primary
• culture

[thesystemsfailed]

Is that really fully 3d? I didn't think they structured along such a flat plane. Its interesting if they are, I guess it would be far more organized.

[DocBones]

@thesystemsfailed: The neuron appears flat (and 'squashed) because it was grown in a culture dish; neurons grown in dishes tend to flatten out against the dish and substrate to which they adhere. The true '3D' in vivo context is, unfortunately, lost in cell culture.

[cutiephotographer]

Was that live or fixed?

[DocBones]

@cutiephotographer:

The hippocampal neurons were fixed. Live would have been really, really nice, but . . . well . . . you can't have everything! ;-)

Thanks for the query!

[DocBones]

These movies are made from pseudo-confocal image stacks acquired on a Leica DMIRB-E inverted fluorescence microscope. Deconvolution and reconstruction were performed using the image acquisition and analysis software package Slidebook 4.0 (Intelligent Imaging Innovations, Inc., Denver CO). DAPI was used to label nuclei.

[tzeqin]

@thesystemsfailed - but less flexible... read: "plasticity"

[bin4z]

Flat and squashed.

[simsima34]

C'est fait par microscope à déconvolution?