Thawing Cells: GIBCO® Cell Culture Basics

Cells are stored at liquid nitrogen temperatures because the growth of ice crystals is retarded below -130°C. To maximize recovery of the cells when thawing, the cells are warmed very quickly by placing the tube directly from the liquid nitrogen container into a 37°C water bath with moderate shaking. As soon as the last ice crystal is melted, the cells are immediately diluted into prewarmed medium. The thawing procedure is stressful to frozen cells, so using good technique and working quickly ensures that a high proportion of the cells survive the procedure. We recommend that you closely follow the instructions provided with your cells and other reagents for best results. This video presents the best way to thaw cells without harming them in this stressful process. Our scientists demonstrate how to carefully transfer cells from storage in liquid nitrogen to the incubator.

The entire Cell Culture Basics training course is available online at http://www.invitrogen.com/cellculturebasics. The handbook and videos provide an introduction to cell culture, with a focus on maintaining cell health throughout the processes of culturing, freezing, thawing and passaging cells. The handbook also contains troubleshooting guides, protocols and product recommendations that researchers at any experience level will find helpful. You want to ensure that you and everyone in your lab is using best practice cell culture technique. Cell Culture Basics can help.

Category:

Science & Technology

Tags:

• cell thawing
• cell culture thawing protocol
• cell preservation
• cryothawing
• thawing frozen cells
• thawing cells

[VideoGameCoupons]

super like your video presentations!

[kiwicutify]

Should you first tap/flick the tube to dissociate the cell pellet before adding media and transferring to a new flask?

[chroniclerofthe70s]

@yoelco Are you trying to be a wiseguy? Lab coats are seldom used. This is because antibiotic and antifungal reagents are added to the cell culture media. Asceptic techniques also keep contamination of cell plates to a minimum. Have you ever conducted scientific research requiring cell culture techniques?

[yoelco]

@chroniclerofthe70s well, guess you ud better keep the coat clean,.

[chroniclerofthe70s]

@yoelco Nonsense, it's a lab coat not a robe. Humans are constantly shedding millions of skin and bacterial cells from skin surfaces.

[yoelco]

ur not supposed to use a robe, more germs and contamination