Do PCR Better than Gradient - A Stop-motion Animation

Over 6,000 PCR-tubes and 30 plates were used to make this stop-motion animation. The video imagines a day with gradients, and how the Veriti® thermal cycler allows you to do PCR better than gradient.

See the "Making Of" video here: http://www.youtube.com/watch?v=b41BX13HXzk

With the Veriflex® technology you can independently set each of the 6 individual zones rather than just set the high and low temperatures of a gradient. This means that optimization of a new reaction can be done using 6 precise temperatures, (giving the user more freedom to try varying annealing temperatures) and you can effectively run up to 6 separate thermal cycling protocols on the same instrument at the same time.

For more information, go to
http://www.lifetechnologies.com/veriti

[audio transcript]

So I'm doing gradient PCR, trying to optimize the annealing, and I need to find the precise temperature. But instead of setting a range, I wish I could set specific temperatures at the same time. And then I get to imagining: Imagining a day with gradients and no precision. What it would that be like?

It would be like: In the middle of my dreamy slumber of my paper being published in Cell, I'm woken - and I don't know whether I overslept or not - because my alarm clock can't be set at a precise time! And then I get in the shower - and try to find the right mix of hot and cold, but I'm just not able to get the temperature right!

It would be like: Making my breakfast. And yet no matter how hard I try, I just can't get the perfect toast!

It would be like: Heading off for a much needed coffee at my favorite coffee shop, and I'm not able to order the right size! So with a coffee so ridiculously big it needs a straw, I head for my car. I get in. Take off. But I have only a vague idea how fast I'm going! Then I find a place to park. Pull up. But again there's this gradient thing going on, and I can't be precise about of time I want to spend here!

It would be like: Getting in the lift and I'm just not able to find the right floor! So imagine if I could actually set the lift to 6 exact floors all at the same time??!

And that's precisely what I want to do with my PCR! Instead of setting the high and low temperatures of a gradient, now I would actually be able to set a number of precise temperatures!

Well : WELCOME to a Life BETTER THAN GRADIENT!

Veriti® Thermal Cycler with Veriflex® Blocks

- 6 temperature blocks
- Set individual temperatures for each block
- 6 accurate temperatures for better PCR optimization
- Combine up to 6 different conditions in one PCR run
- Precise incubations for non-PCR workflows

For more information, or to request a demonstration of the Veriti® thermal cycler in your lab, please contact your Life Technologies representative or complete the Contact Me form at www.lifetechnologies.com/veriti

Category:

Science & Technology

Tags:

• Veriti
• Gradient PCR
• Annealing temperature
• PCR Optimization
• Gradient Thermal Cycler
• pcr song
• Ph.Diddy
• jelly bean song
• Veriflex
• PCR
• Thermocycling
• Annealing
• stop motion
• thermal cycler
• stop motion animation
• polymerase chain reaction
• DNA sequence
• applied biosystems
• life technologies

[LifeTechnologiesCorp]

Yes, it's true to the art stop-motion animation. It took weeks of moving over 6000 PCR-tubes and thousands of photos to create the video which tells our story. That is, you wouldn't want to live in world of gradients, so why should your PCR have to?

[Athstman]

@JjmmyM It stands for Polymerase Chain Reaction. It is a process to take an existing amount of DNA and place it in a solution with the enzymes that are in the nucleus of a cell. The enzymes then replicate the existing DNA, allowing you to have much more for experiments.

[rogeriomg20]

Great!!! Very nice....... I'll remember this video every time I'll use a PCR tube \o/

[BarteczQus]

trolololololololololo trooolololololo

[AutoTuneThat]

looks like a light bright lol

[JaKanskjeNei]

Very cool! Surely this must be computer generated - is this really stop motion??

[sasosore]

@JjmmyM Polymerase Chain Reaction

Basically its used to amplify a section of DNA by having it copy itself numerous times, until there's enough copies of the DNA strand to get detectable results. The resulting DNA can then be sequenced or used to tell if you have a certain gene.

[JjmmyM]

This is interesting, but I've no idea what a PCR is.