Sorry but a random endonuclease makes the cut if there are no damages and exonuclease degrades the 5' region. Then Rec A guides the single 3' strand to the homologous strand. Then the open loop acts as a template for DNA synthesis since Dna Polymerase cannot synthesize DNA from scratch. Then branch migration occurs and forms a heteroduplex. Then isomerization which makes the double helix twist, starting uncrossed then the intermediate occurs creating a holliday junction and finally crossover.
Once the process is complete it undergoes resolution. which means it just cuts the two strands using endonuclease
joshuavillazana 4 days ago
Sorry but a random endonuclease makes the cut if there are no damages and exonuclease degrades the 5' region. Then Rec A guides the single 3' strand to the homologous strand. Then the open loop acts as a template for DNA synthesis since Dna Polymerase cannot synthesize DNA from scratch. Then branch migration occurs and forms a heteroduplex. Then isomerization which makes the double helix twist, starting uncrossed then the intermediate occurs creating a holliday junction and finally crossover.
joshuavillazana 4 days ago
Good, I like that you share this video, I wish success always Homologous Recombination of Double Strand Breaks
jhoenedu 1 week ago
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jutsmyface 1 week ago
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godguetar 1 week ago
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indiage 1 week ago
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WaelYakti 1 week ago
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shariarahman 2 weeks ago
This is useful! :)
12kwh 3 weeks ago
amazing!! but its exonucleases not endo
m7MD292 1 month ago