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Agilent's Stratagene Real-Time PCR (qPCR) System

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Uploaded by on Mar 3, 2009

For all of your QPCR needs, and more, the four- and five-color Mx3P QPCR systems are the perfect choices, giving you the most flexible, complete solution at the best price.

Stratagene offers you the complete QPCR system solution with high-performance, value loaded products and services to support your QPCR research. Whether a novice or experienced user, our goal is to ensure that you are running QPCR experiments quickly, efficiently, and affordably. Our Mx™ family of QPCR Systems, premiere QPCR System Service Program, complete line of QPCR and QRT-PCR reagents, and Fast Track QPCR Education Program is the total package for your QPCR research.

Instrument:
Excitation source: Quartz Tungsten Halogen lamp
Detector: 1 scanning photomultiplier tube (PMT)
Multiplexing capabilities: 4 or 5 targets in the same tube
Thermal system: solid-state, Peltier-based
Embedded computer safeguards data
Filters: Dual customizable filter wheels with four or five user-selected filters with independent filter control.
Tube/plate formats: 200ul, 96-well format, optimized for 25ul sample size
*Choice of filters includes: FAM™/SYBR® Green I (492nm-516nm), TET (517nm-538nm), HEX™/JOE™/VIC™ (535nm-555nm), Cy3™ (545nm-568nm), TAMRA™ (556nm-580nm), ROX™/Texas Red® (585nm-610nm), and Cy5™ (635nm-665nm).

Also included: notebook computer, MxPro™ QPCR Software, Installation and Setup Guide, Introduction to QPCR Guide.

System Specifications:
Dynamic Range: 10 orders of magnitude
Excitation Range: 350nm-750nm
Emission Range: 350nm-700nm
Temperature Uniformity: +/- .25°C at 72 ˚C
Temperature Ramp Rate: up to 2.5°C/second
Temperature Range: 25-95°C
Dimensions: 13¨ (33cm) W x 18¨ (46cm) D x 17¨ (43cm) H, Weight: 44 lbs (20 kilograms)

MxPro™ QPCR Software:
Powerful, flexible, easy-to-use
Perform multiple sub-experiments on a single plate
Real-time amplification plots viewed and results analyzed during run
Standard curves plotted for multiple dyes on a single chart
View all dyes on one amplification plot, or view each single dye in multiplex experiments
View and analyze data in multiple formats including amplification plots, scatter plots, fluorescence intensity screens, final call results, melting curves, annealing range, and text reports

All results available as raw data. Easily export data to Excel, PowerPoint and text files.

Flexible analysis options: amplification plots, scatter plots, standard curve charts, fluorescence intensity, final call results, melting curve, annealing range and text report
View all dyes for multiplex experiments on one amplification plot and/or standard curve chart or view individual plots for each individual dye
Melting curve analysis with automatic melting temperature (Tm) calculation
Allelic discrimination by threshold cycle or by endpoint fluorescence values with auto-call feature
Export chart and text report data directly from display screen to Microsoft® Excel, PowerPoint, text and image file
Software can link up to six (6) Mx3005P and Mx300P instruments with one computer

The Mx3000P® and Mx3005P® QPCR Systems are authorized real-time thermal cyclers.
This are Licensed Real-Time Thermal Cyclers or Licensed Real-Time Temperature Cycling Instruments under ABI's United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics. No right is conveyed expressly, by implication or by estoppel under any other patent claim.

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