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KIR typing by PCR-SSP: tips for critical steps

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Uploaded by on Feb 9, 2011

This video shows two critical steps in the process of KIR-gene typing by PCR-SSP:
1-How to prepare 24 ready-to-use typing sets
2-The rapid loading of six typing assays into an agarose gel, with the help of an 8-channel pipetting device.

Further technical details published in:
1.-Vilches et al. Tissue Antigens 2007, 70:415-422. DOI: 10.1111/j.1399-0039.2007.00923.x

2.-Ordóñez et al. In Immunogenetics: Methods and Protocols (eds.: FT Christiansen and BD Tait). Methods in Molecular Biology. Humana Press (in process of publication, 2011)

contact: camycvilches-video@yahoo.com

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