Glowing heart and blood in a fish embryo

Microscope views of a transgenic (genetically modified) zebrafish embryo, 3 days old. Fluorescent proteins are used to make the heart glow green or red (to identify the transgenic fish) and the red blood cells glow green. Notice the magnifying effect of the lens in the eye (black circular thing in the top of one of the views) as red blood cells travel through the retina

Category:

Science & Technology

Tags:

• heart
• blood
• GFP
• RFP
• transgenic
• zebrafish
• embryo
• developmental
• biology
• vessel
• aorta
• circulation

[ParaglidingManiac]

That's very very impressive. How small is it? what magnifications did you use?

[kjpg2005]

@ParaglidingManiac

Can't recall the magnification. No more than 200x. The heart is probably around 100µm across (0.1mm). Glad you liked it.

[ParaglidingManiac]

@kjpg2005,\

Another question. How did you mount your camera to capture this footage, please?

[kjpg2005]

@ParaglidingManiac

the movie is taken on a compound,upright microscope and the camera is attached to the microscope head through a dedicated attachment.

[MissLilPopstar]

a genetically modified fish?does it go through mutation?or is it a cross bred fish?

[blubbiblubblubb]

lol youll totally burn in hell! ;)

[rauldacosta24]

excelente vídeo 0.0

[kjpg2005]

Oops.  guess I fail.

[aisles23]

I think you missed the point that they are probably the easiest oviparous fish to reproduce in captivity :P

[kjpg2005]

They are good for genetic analysis (birth defect kind of stuff), are transparent, so you can see the kind of stuff in the movie without dissecting or killing the embryo, and they can be manipulated to add genes or cells, or take them away.