Semen analysis is an important but labor-intensive routine for evaluating male fertility and for preparing artificial insemination. In this video, we demonstrate automated semen analysis using a lensfree on-chip microscope. This compact holographic microscope weighs ~46 grams and does not require any lenses, lasers or other bulky optical components to achieve phase and amplitude imaging of sperms over ~24 mm square field-of-view. Such a field-portable semen analysis platform that can automatically investigate both sperm concentration and motility is especially important for fertility clinics, personal male fertility tests, as well as for field use in veterinary medicine such as in stud farming or animal breeding applications
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