Illumina Solexa Sequencing

An animation describing the process of next generation sequencing on the Illumina platform.

Category:

Science & Technology

Tags:

• Illumina
• Solexa
• Next
• Generation
• Sequencing

[schroedingers84]

Hi Draven,

What program did you use to create the animation? I love this video and I want to try to emulate.

[Draven1983101]

@schroedingers84 I used a piece of software called 3D Studio MAX but there are other options like Maya, even some open source projects like Blender 3D. The learning curve for these programs can be somewhat steep but there are plenty of internet tutorials around.

[issy2010821982]

Hey Draven. I'm not sure how often you check this. I'm presenting my M.Sc. thesis on SNVs identified in CLL and I only get 15 mins to present so I was looking for a video explaining the Illumina platform. This video is perfect for getting the idea across quickly (although it would have been better if it went on to explain four-colour cyclic reversible termination). Do you have a file version or a downloadable version you could point me to, as I do not want to rely on the internet.

[Draven1983101]

@issy2010821982 Sorry for delayed reply. I had a look if I still had the rendered filed and it seems I deleted them when I was having HD space issues. I still have the original modelling files so I would need to re-render and re-edit the animations. When is your presentation?

[Draven1983101]

@VaticanIscariot, Think Sanger sequencing times a million. In a standard sanger run you get about 1000 base pairs (nucleotides) of sequence, from a single lane on an illumina GAIIx you get about 1.2 billion bases. So I won't say it's better than sanger sequencing because sanger is still useful for small things and even for validation of next generation sequencing, but in terms of throughput NGS is incredible.

[Draven1983101]

@asoaoj Automated Sanger Sequencers are the first generation of sequencing technology. Illumina/454/Solid are part of whats called next generation sequencing (NGS) or ultra high throughput sequencing technology. The major difference is in the parallelisation, automated sanger sequencing allows you to obtain a single read of around 1000 base pairs from a reaction, NGS returns upwards of 30 million reads of around 100bp from a single lane, meaning you get 3 billion bases instead of 1000.

[schroedingers84]

I checked them out, very helpful, thanks!

[sallysam164]

Thank you for the amazing video! really appreciated :)

[AkiraYC88]

Question, are the adapters ligated to both ends same?

[issy2010821982]

@Draven1983101 It's ok. I couldn't fit it in anyway due to time restraints and I found a video on the Illumina site that I can pull up if I get any questions on it.