Lec 10 | MIT 5.301 Chemistry Laboratory Techniques, IAP 2004

Column Chromatography

It takes considerable practice to master the art of "running a column". This video will get you started, with tips on picking appropriate conditions, packing and running a column, monitoring separation, and even making a micro-column from a pipet.

View the complete course: http://ocw.mit.edu/5-301IAP04

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Category:

Education

Tags:

• mit
• opencourseware
• chemistry
• laboratory
• purification
• extraction
• transfer
• chromatography
• experiment
• spectraoscopy

[mrpopeye17]

Great video... I have an exam on columns this afternoon and this really cleared some things up!

[steeeee33z]

debussy arabesque no.1

[hairytoothgaming]

Great video! Thanks for sharing.

[ChemistDrummer]

You forgot to add another "Caution!". Never clamp the pressure tube in place when having the stopcock close like shown in the vid. Danger of explosioni if you dont release the pressure quickly enough.

[cyclestring]

great vid. . I learned a lot about column chromatography. . thanks pal! :)

[Bakugantsuvai1]

The way she shows us the video is so funny

[dianafeiteira]

fml i dont undrestand nothing about this...im so screwed :(

[bassmanjr14]

use heptane, better for the environment

[lexichronicle2]

"There are also columns available with sintered discs at the base to avoid the cotton and sand part"

Speaking of which, I was just watching a column video that involved such a sinter, and the top of the column was bent, whereas this one is flat. indicating that care can overcome better equipment.

[Silverweed]

Having to collect 100+ fractions and spot all of them on TLC only to realize you didn't get enough separation. Having the column fissure right after adding your precious sample and having to recover every bit you can rotovapping down tons and tons of solvent praying you can get everything back. ughhhh never again please!! :P