2011 02 04 NYU CHIBI Proteomics Workshop - Part 1 David Fenyo

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Uploaded by on Nov 23, 2011

This workshop will give an overview of proteomics and mass spectrometry workflows, experimental design, and data analysis. The following subjects will be covered in three lectures: (1) Protein identification (peptide mass fingerprinting, tandem mass spectrometry, database searching, spectrum library searching, de novo sequencing, significance testing); (2) Protein characterization (protein coverage, top-down proteomics, post-translational modifications, protein processing and degradation, protein complexes); (3) Protein quantitation (metabolic labeling - SILAC, chemical labeling, label-free quantitation, spectrum counting, stoichiometry, biomarker discovery and verification). Examples will be provided throughout the course on how the different approaches can be applied to investigate biological systems.

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