Next Generation Sequencing

What does the future hold for genome sequencing?

The Human Genome Project was more or less completed in 2003. Current projects are underway to use that initial exploration to create more useful post-genomic tools for discovery. What tools will be used, and how do they compare to the previous generations of technology?

This is a video that I've wanted to make for a long time. If you'd like to see me make more "cool biotechnology" videos, let me know in the comments.

Category:

Education

Tags:

• DNA
• sequencing
• next-gen
• human
• genome
• project
• 454
• Life
• Sciences
• Roche
• SOLiD
• Illumina
• science
• molecular
• biology
• c0nc0rdance

[GingerBandit13]

Thank you for the wonderful video on Next Gen. Sequencing-- a brilliant example of building on the shoulders of giants. I especially enjoy how this method is reminiscent to the methodology of Sanger Sequencing. Do you have any information about the error rate of this process? I image the PCR would introduce some at a certain level during the cluster formation...

[C0nc0rdance]

@GingerBandit13

PCR has a de novo mutation rate. The sequencing has a false base substitution rate...

This paper suggests that:

PCR unamplified error rate is 0.05% of bases

PCR amplified error rate is 0.25% of bases.

Nucleic Acids Res. 2010 November; 38(21): 7400–7409.

It's typical to represent every sequence used in 4-8 independent reads, called the "sequencing depth".

[Psycatalyst]

Ion torrent is currently the fastest sequencing machine detecting the pH of base pairs. This is faster than the cost of the 454 in reagents which is ~$8000.

[C0nc0rdance]

@Psycatalyst

I hadn't heard of this tech until last week. Apparently they have been acquired by Life Tech aka Applied Biosystems/Invitrogen (a company I despise). Will they keep both the SOLiD and PGM, do you think?

[celshader]

There seems to be an exponentially growing gap between those who understand science and those who don't.

[StopSpamming1]

Totally amazing what the human brain in conjunction with 2 hands that are not clasped in prayer can achieve.

[kylis22]

the more technology, the easier for the few in power to control the masses

/watch?v=eAaQNACwaLw

[Dewkeeper]

Do you think you might make more "Cool biotech" videos? I really liked this one.

[Ekergaard]

Great video. About the last, I admit that I have gotten afraid of new knowledge, for example epigenetics. Very simplified, perhaps I can express it like this, genetic determinism appeals to me, however "fans" of epigenetics do not believe in genetic determinism. So epigenetic scared me, since it challenge something that appeals to me. But I will of course not reject epigenetics or anything else, just because I'm frightened by the idea

[iluan666]

@iluan666

* I ment thaks for giving us all This information.

[iluan666]

C0nc0rdance. As an undergrad biology student, who aspires to become a researcher in the field of molecular biology, I must tell you that I admire you deeply and I consider you an inspiration and a role model. To me you are like a template of what I want to become. Thanks for giving us all information.

[lorddreams469]

@celshader I totally agree! I think that part of the problem is that science isn't being adequately taught in many classrooms. I didn't fall in love with science until I was in college getting a degree in business (long story). Another issue is that many scientific disciplines are evolving so rapidly, and becoming evermore complex, that some scientists, not to mention most laypeople, can't keep up. Then, rather than tying to learn and understand, they just go by what someone else says. Sad.

[lorddreams469]

@rraassll Reconsider not giving the thumbs up. I think that based on the Next-gen info provided, a thumbs up is deserved. I too am a religious person; I believe in creationism, and I'm working on a PhD in molecular cell biology. I don't understand how my beliefs "cheapen" the science I work on. Whether or not I believe in God has no bearing on what makes up the physical world around me. I don't know what the last minute added to the scientific process understanding, but it's his video. :-)

[attheveryend]

BORE ME WITH THE DETAILS!

PLEEEEAAAAAASSSEEEE!!!