Polymerase Chain Reaction (PCR)

Polymerase chain reaction (PCR) allows researchers to amplify DNA in a test tube. This process uses an enzyme derived from heat-resistant bacteria. The steps of PCR are driven by changes in temperature.
Originally created for DNA Interactive ( http://www.dnai.org ).
TRANSCRIPT: Polymerase chain reaction (PCR) is a process where many copies of a specific piece of DNA can be made. This is known as amplification. Double-stranded DNA (red) unwinds and separates when the temperature is increased. As the temperature is decreased, small starter sequences called primers (glowing) can attach or anneal to the DNA. These primer sequences are usually only 20 to 25 nucleotides long, and are designed to match the start and end points of the DNA piece to be amplified. Once the primers have annealed, Taq polymerase (blue) copies the DNA starting from the primer. The temperature is increased; the strands separate; more primers anneal; the DNA is copied; and this cycle is repeated many times. In a typical PCR reaction there are 30 cycles, which can potentially create one billion copies starting from one molecule of DNA.

Category:

Education

Tags:

• polymerase chain reaction
• polymerase chain reaction pcr
• dna primers
• double stranded dna
• manipulation techniques
• pcr reaction
• dnai
• nucleotides
• chain reaction
• one billion
• amplification
• strands
• molecule
• sequences
• animation

[salunete]

oh, so that's what they mean by amplified :)  Only the desired portion is copied!

[FateStayNightOST]

The DNA is heated, denatured into two separate strands, 15 to 20 nucleotides are present in which they act as primers on the and 5' and 3' end, by doing this there is a middle section. Heat-resistant polymerase (from bacteria from the hot springs) does away with the rest of the dNA strand. The frequency at which a wanted sequence is easy to get by multiple replications. 3/25%, some issues are base pair mismatches or errors.

[Basiriku]

Not as informative as could be, but really beautiful

[zackboomer]

@beamoflaser oh, the obvious "you care because you replied" response. I don't give a shit about german because it will never be useful for me.

[beamoflaser]

@zackboomer you probably did because you replied. But keep thinking you're a cool dude because you don't care about stupid stuff like languages.

[zackboomer]

@MilezJoBroluver94 lol you think I care that I wrote some stupid language wrong?

[MilezJoBroluver94]

awesome video thank you! perfect for my Biology exam... 

[MilezJoBroluver94]

@zackboomer try to translate on your own not via google traslator... you kinda... wrote everything wrong!

[MilezJoBroluver94]

@zackboomer that was the german word for Biology... Biologie

[Ebeet0FAN]

@isam1335 a synthetic one