H&E Staining

( http://www.abnova.com ) - H&E stain is a popular staining method in histology. It's a combination of two dyes: the basic dye (hematoxylin) and the alcohol-based dye (eosin). In an H&E stain you'll usually see both eosinophilia and basophilia: the nuclei of cells basophilic (blue), while eosinophilia is typical of cytoplasmic constituents (pink). More videos at Abnova http://www.abnova.com

Category:

Science & Technology

Tags:

• H&E
• Staining
• Abnova
• antibody
• protein
• cell
• biology

[txdude86]

5 min in xylene is all u need to get rid of the paraffin and just a min each in the other reagents

[pauldamedtech]

That procedure is too long,and I've notice in this video that no diferentiation with 0.5% HCL after hematoxylin was done.

[ASSHOLELA]

why that guy didnt differentiate or blue?

[TheRealHarsjan]

@MrJazkiller oo ok thanks very much indeed :-)

[MrJazkiller]

@DerSchnurrbart yes i agree.

[MrJazkiller]

@TheRealHarsjan after removing xylene...you need remove the alcohol from the tissue and replace it with water..so that your stains will have more affinity to the tissue. how do you do this? by passing through series of high concentrations of alcohol ( starts from your absolute to remove xylene) then to 90-80-70 then finally to water...then you can start H& E staining.

[MrJazkiller]

@TheRealHarsjan for "Dehydration" alcohol starts from lower concentration to higher then to absolute concentration. Rationale: if tissues are submerged directly to high alcohol con. tissues will become hard or brittle, the outer portion of the tissue will dehydrated quick but the inner part is not fully dehydrated.

Prior in performing H & E staining, you need to " Deparaffinize " use xylene, then remove xylene by absolute alcohol ( xylene is only miscible w/ absolute alcohol )

[Azander137]

Is this how babies are made

[TheRealHarsjan]

I know that the alcohol is used for dehydration, but why so many different types of concentration? (student :) )

[melonshower]

Very nice!