Yay, Science!

A short presentation on some of the things I'm doing in the lab =)

http://en.wikipedia.org/wiki/Gel_electrophoresis
http://en.wikipedia.org/wiki/Native_PAGE
http://en.wikipedia.org/wiki/Nitric_oxide
http://en.wikipedia.org/wiki/Antioxidants
An excellent literature review written by Pacher and co-authors titled 'Nitric Oxide and Peroxynitrite in Health and Disease' is also available. No journal subscription is required to view.
http://physrev.physiology.org/content/87/1/315.full.pdf+html




While I have received some vote of confidence from viewers concerning their willingness to donate to my proposed cause, a major concern is transparency and how exactly the raised funds will be transferred to the recipient. No doubt finding a solution to this problem will increase support for this cause. I've recently communicated with a representative from Rotary International and I am beginning a three-way conversation between myself, them, and the campaigners in Africa. Ideally, donors will simply give donations to Rotary, and they in turn will hand it over to Dr. Uzoegwu and affiliates. I hope things will not become too complicated for myself. My next video will most probably be related to this issue in one manner or another.

I started my research into nitric oxide and antioxidant enzymes some 16 months ago when I was a student working on my undergraduate thesis (which I have already completed). Throughout this time I have been perfecting the procedures used throughout these experiments, and the results show that I may have a publication in the making. As my supervisor is incredibly busy, and as I know the experiments better than he does at this point, I've volunteered to help train the new thesis project student in our lab, who has started contributing his own data sets, along with a third whom I'll help train starting next week. Research developments would be happening more frequently, though unfortunately family matters have been consuming my time, thoughts, and energy. My mother has recently been diagnosed with terminal brain cancer (CT scan discovered OVER 20 tumours in her brain), and is expected to die within the next couple of months. As my father already passed away some 12 years ago, there will be Wills, property inheritance, and other issues to deal with. While I desire to attend grad school, in light of circumstances, I've decided to take 6 to 12 months off after I graduate in April as there will be far too many decisions and issues for my younger brother and I to deal with otherwise. I'm completely burned out from university as it is, and the extra time (ah, time, art thou one elusive bitch!) will also allow me to develop my research profile so that I will be a more desirable applicant when I do apply for graduate studies. While I find burying myself in laboratory work therapeutic, you may also find me producing some long overdue videos with that free time. Hence, don't expect too much activity on this channel except for updates concerning this upcoming charity until I have my life under control again.

Category:

Education

Tags:

• Laboratory
• research
• science
• biochemistry
• chemistry
• enzymes
• proteins
• nitric
• oxide
• antioxidant
• oxidant
• superoxide
• dismutase
• catalase
• hydroperoxidase
• radical
• molecule
• Escherichia
• coli
• signal
• transduction
• inhibition
• expression
• regulation
• electrophoresis
• sickle
• cell
• anemia
• fetal
• haemoglobin

[mymojorisin]

LOL A lab coat and props does not make a scientist.  What a moron.

[UNFFwildcard]

@mymojorisin

Troll harder next time.

[BlowDevilUp]

What about using microarray technology to determine the "protein expression patterns"?

[UNFFwildcard]

I could, but then I would have to re-develop the methodology from scratch. Simple native PAGE serves me well.

[myjizzureye]

Come on own up, you have been feverishly working on a cure to male pattern baldness that is as so far.....not really bearing much fruit.

[pineconesaresmallnow]

Untreated sample of SOD proof that aliens exist???

[migkillertwo]

yeah mr. white! YEAH SCIENCE!

[NashvilleKildCountry]

@UNFFwildcard

I completely agree on the abstinence thing, and I'm not really religious. I just think it's the only fail-safe preventative measure against STI's and getting someone pregnant. I can kiss my college degree and life ambitions goodbye if I get some girl pregnant. 20+ years is simply not worth the ~20 minutes of physical satisfaction, no matter how supposedly narrow the risks are.

[truvelocity]

My oldest and one of only two brothers died of brain cancer and my Uncle has the same exact brain cancer. I will say, life is a bitch, but as long as we have biochemical engineers and people willing to understand gene expression, we can get closer to finding out what we can do to suppress that expression. I'm not biochemist, but I get enough of the gist to know when someone is making sense or not

[MorphicStates]

This is so far over my head... O.o

[helical4]

Hey, do you know if those who are homozygous for Sickle-cell Haemoglobin and that also express fetal Haemoglobin are immune to Malaria?