The Spread Plate Technique
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i'm student of pharmacy from malaysia...this video is really help me to understand better about technique of isolation :)
ciklola16 11 months ago
As to the comments about his not-so-aseptic technique- For starters the pipette tips are sitting in a jar with an open lid on the bench, which leaves plenty of opportunity for spores and airborne bacteria to settle; which is common. This is why we work in a laminar flow hood, and wear gloves, but this is just a demonstration...
Abraley42 1 year ago
@jenny07la Yes, this is common practice. You only flame the neck of the bottle - to get rid of any contaminates in the air inside the neck... If that makes sense? ^___^
LizzyJaneway 1 year ago
Maybe my comment was a bit too rigorous. At that time I myself was used to do all this in a clean bench. In theory, some microbes could land on the agar plate when he plates the culture on the agar plate, contaminations are quite common if you don't watch out. But in most cases you should be fine.
However, 500 µl on a single agar plate? Plating 200 µl is annoying, let alone 500 µl! You would need a really dry plate (and much time) for that to dry completely...
yeahdude7331 2 years ago
Im new to this...care to explain to me what he did wrong in terms of sterilization? I'd really appreciate it.
foeasy16 2 years ago
I like all your videos, you explain really well, thanks:)
TearsForLosers 2 years ago
I think he doesn't use the concept of sterilization
081908293134 2 years ago
Of course it's neccessary to pass the strain bottles through flame. This will check contamination from the air!
Ankitvkc 2 years ago
i didnt know you have to sterilize the little strain bottle, does anyone know if this is common practice to do this? I suppose the cultures would always be in glass containers, but I'd be afraid id kill the culture by passing it through the flame
jenny07la 2 years ago
I guess some people have a different understanding of "sterile" :-)
yeahdude7331 3 years ago