See the 199 section version of portal tract reconstructions at http://www.youtube.com/watch?v=4GHgS_ORxcw
This video compares the hepatic portal track of a normal dog's liver to a dog with a congenital vascular abnormality termed a portosystemic shunt.
In this condition the portal vein has an abnormal connection to the systemic venous system and deprives the liver of the normal portal blood flow.
Red=hepatic arteries, Blue=portal vein, Green=bile duct, Yellow=lymphatic space.
In the normal portal tract the size and relationship of the portal vein, the hepatic artery, bile ducts and the lymphatic spaces can be seen. The hepatic portal tract from the dog with the portosystemic shunt is characterized by the lack of a portal vein, dramatically distended lymphatic space and an increased size with multiple small branches of the hepatic artery.
This negative-space filling technique was performed on a small number of sections (normal dog=40, shunt dog=48) from FAA-fixed, paraffin-embedded liver tissue. Paraffin blocks were sectioned at 10 microns and treated with a standard H&E stain.
Sections were photographed with a Sony DSC707 consumer-grade camera on a Nikon Eclipse 50i at 200X. JPEG images were imported into Adobe After Effects on a Macintosh desk-top computer. Details of the process used to build the 3D volumes are described in a recently published article: http://www.ars.usda.gov/SP2UserFiles/ad_hoc/66452500ResearchSpotlight/Livings... and in our other video: http://www.youtube.com/watch?v=zlw-50RHoec&feature=youtube_gdata and
The 3D images are not cartoons but represent the actual empty space inside the vessels in each image. The technique is ideally suited for tissues that are too large for analysis by Confocal Microscopy but too small for MRI or PET. In addition, the cost of analysis is negligible, since it uses a consumer-grade camera on a conventional light microscope and off the shelf software by Adobe.
For more examples of 3D reconstruction using this technique visit: http://www.ars.usda.gov/Main/docs.htm?docid=19570
For more information on diagnosis of liver diseases in dogs and cats, using both histological and clinical criteria, see the monograph: "WSAVA Standards for Clinical and Histological Diagnosis of Canine and Feline Liver Diseases" http://www.elsevier.com/wps/find/bookdescription.cws_home/708062/description#...
Distortion-free sections provided by Research Associate Tan Tuong. Graphics Consulting provided by Randy Gelman Beltline Media http://www.beltlinemedia.com
Contacts: John Cullen (VMD, PhD, Professor, Veterinary Pathology, Faculty of
Population Health and Pathobiology, College of Veterinary Medicine, North
Carolina State University, Raleigh, NC, USA) john_cullen@ncsu.edu
David Livingston (PhD, USDA-ARS Research Agronomist, Professor, Department of Crop Science North Carolina State University, Raleigh, NC, USA): dpl@unity.ncsu.edu.
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Cryostat microtomeby IBVU4U2,083 views
I just found what i was searching for. You made a great animation . Silently you have proven that you don't need a big mouth those who talk a lot but explain little.
amshuman100 7 months ago