Southern Blot

No description available.

Category:

Science & Technology

Tags:

• Southern
• Blot

[kingkonghitme]

is gel electrophoresis and southern blot the same thing ? :S

[ThePlantGenetics]

@kingkonghitme Gel electrophoresis is one of the steps you go through when you do Southern blot.

[AgentMultiSuccess]

can someone tell me if the DNA in this technique is single stranded or double stranded....PLEASE! :)

[ThePlantGenetics]

@AgentMultiSuccess the DNA is double stranded until the electrophoresis step. after finishing the electrophoresis, you treat the gel with solution to denature the double strand and when you do the blotting on a membrane, it will stay single stranded for the hybridization to take place.

[HuSnaMujahidah]

morning. i have a litle bit confused about the mechanism occur during using the electrophoresis... is that true that the smaller size will migrating faster than the larger? bcoz i thouth that the larger size will migrating faster as it is dense rather the smaller size.. any body help me please?

[ThePlantGenetics]

@HuSnaMujahidah Bigger fragments find it difficult to pass through the tiny pores in the gel. smaller fragments can pass easily. That is why smaller fragments travel faster than the longer ones.

[jccarbunkle]

@AgentMultiSuccess I think its single stranded. If it were dsDNA the radioactive probe wouldnt be able to bind

[MrNgwachii]

the DNA is single stranded in order to allow hybridization, the smaller the DNA fragment the faster it migrates during electrophoresis. larger DNA fragments move slowly through the gel :)

[sagh2787]

simple and clear

[FIEKESAYSHELLO]

uhm... does that mean that person B has a deletion ? or ...