Gas Chromatography GC

An education video from the Royal Society of Chemistry on gas chromatography using a flame ionisation detector (FID) with a brief mention of gas chromatography mass spectrometry (GCMS). From the "Modern Instrumental Techniques for schools and colleges" DVD. For more information on the Chemistry for our Future programme please visit http://www.rsc.org/CFOF (C) Royal Society of Chemistry

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[shoukatkhan01]

Hi,

yes in GC the separation of analyte depends on its volatility but we should keep in mind that more polar the compound less volatile it would be,second thing the polarity of column in GC is also very important bcz inside the column the analyte molecules do not travle in a very straight line they move randomly and when they come in contact with the walls of coulmn they stay there for a while depending upon the polarity of analyte and column.

[traxass000]

i got a question here. so GC has nothing to do with the polarity of the substances used in order to separate them (because my teacher keeps on saying the polarity plays an important roll, like the more polar particles remain behind while the rest goes through the tube) ? because here and on other videos i here people mention only volatility of the substances as an important factor. :S :-? need explanation asap help help :p:p its for a project and great vid btw ;)

[BuickDoc]

@shaggyburns One method I used is called preparative gc. A larger column and a larger volume of injectate is used. As the carrier gas exits the machine, dry ice cooled traps are inserted in the gas stream. Any liquids are thus trapped. Change the trap for each peak on the chromatogram, to isolate each compound. They can then be identified by standard methods, ie IR, NMR, Mass Spec, etc. Another method is to use a GC/Mass Spectrometer. High resolution MS will usually give a unique structure.

[callmehoo]

Hello. I want to ask about GC TCD-thermal conductivity detector can u show a video about it?

[callmehoo]

@ncpatch If a liquid is viscous that implies that the liquid has resistance to flow. For example petroleum gel and water, which is more viscous? The answer is petroleum gel as it flows more slowly than water across a slanted plane.

[ncpatch]

Any one know what viscous means?

[bonny247]

hii friends can any one give me information on what compounds can be dervitized??? and what is the exact method for sample extraction with the use of derivatizing reagents?? lidocaine, procaine mesalamine bupivacaine topermate can any of these be dervatized??

[crudeoilsystems]

very nice. Thanks

[hplcflowmeter]

thanks

[shortie177]

@shaggyburns you need to use the external, internal standard or standard addition method of anlysis to determine which peak is which if you have unknowns.

[shaggyburns]

how do you know which compound each peak is?

[asteriscotriangulado]

Awesome! :D