Calcium Wave During Fertilization
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8:16
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That B just got Hiroshima'd
TheBsubtle 1 year ago
Any advice will be appreciated (v.beltran@aims.gov.au)
Cheers
VB
vicobeltran 1 year ago
For example in vitro after bioluminescence reaction, aequorin can be regenerated via EDTA and reducing agents to again bind calcium and produce photons, but I don’t know what happens in vivo, whether the cell have heaps of AEQ and deploys some of them every time the neural impulse release calcium or there is a mechanism of calcium detachment from the EF-hand proteins once they are no longer required
vicobeltran 1 year ago
I am not an expert in Calcium signalling but I would like to know what happen with the calcium ions AFTER bound to the target protein: either a) they are sequestered by a chelating compound and then returned to the endoplasmic reticulum or b)they remain bound to the target protein (ei. Calmodulin, aequorin) and then the whole protein is recycled via ubiquitin pathway?
vicobeltran 1 year ago