Semi-Automated ELISA Liquid Handling

This video depicts the liquid handling process steps of a typical ELISA carried out using EL406 Combination Microplate Washer Dispenser from BioTek Instruments. The video follows the process of a single plate from start to finish, which was automated using a BioStack Microplate Stacker to supply plates and serve as an incubation storage location. Both devices, as well as necessary timing control, were maintained using Liquid Handling Control (LHC) software. Note that the blue coloring of solution in the microplates initially depicts the samples and standards having been loaded onto the plate already. The first step is a wash to remove unbound material followed by a reagent addition (red solution). The plate is then returned to the BioStack for incubation, after which the plate is moved back to the EL406 for a second wash to again remove unbound material followed by a second reagent addition (yellow solution). After incubation, a final stop reagent is added using the EL406 (blue solution) that, when added to the microplate wells, halts the reaction. The plate is returned to the BioStack and would then be manually moved to a microplate reader.

Visit http://landing.biotek.com/el406-em to learn more about the El406 Combination Microplate Washer Dispenser.

Category:

Science & Technology

Tags:

• automated
• elisa
• process
• liquid
• handling
• biotek
• instruments
• microplate